Chr2 h134r -eyfp ai32
WebR-134a Refrigerant; UV Leak Detect; Self-Seal Can; 12 Ounce; Freon (TM) 134a plus UV leak detector helps save mobile AC professionals time and money by combining the … WebDownload scientific diagram Western blot analysis of ChR2 (H134R)-EYFP expression in Ai32 mice. Olfactory bulb (OB) cell lysates were immunoprecipitated using a rabbit anti …
Chr2 h134r -eyfp ai32
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WebIn addition to the viral particles, you will also receive purified pAAV-Syn-ChR2 (H134R)-GFP plasmid DNA. These AAV preparations are suitable purity for injection into animals. … Web024109 B6.Cg- Gt(ROSA)26Sor tm32(CAG-COP4*H134R/EYFP)Hze /J Ai32 mice express an improved channelrhodopsin-2/EYFP fusion protein following exposure to Cre …
WebMay 1, 2024 · Ai32 and Ai39 mice were crossed with Scn10atm3(cre/ERT2)Jwo mice to generate offspring expressing ChR2(H134R)/EYFP or NpHR3.0/EYFP, respectively, in Nav1.8+ primary afferents. At 6-8 weeks of age, female heterozygous mice were anesthetized and instrumented with a transurethral catheter. A fiber optic was passed … WebMar 16, 2016 · Immunostaining for ChR2-(H134R)-EYFP. One GlyT2-Cre; Ai32 mouse was transcardially perfused with 4% PFA, and the brainstem part containing DCN was dissected out for postfixation. After postfixation, the tissue was sectioned at 30 μm thickness, washed in PBS for several times, blocked in BSA, and incubated in a rabbit polyclonal anti-GFP ...
WebPlasmid pAAV-hSyn Con/Foff hChR2(H134R)-EYFP from Dr. Karl Deisseroth's lab contains the insert ChR2(H134R)-EYFP and is published in Nat Methods. 2014 Jul;11(7):763-72. …
WebPlasmid Ai27 from Dr. Hongkui Zeng's lab contains the insert CAG-Floxed hChR2(H134R)-tdTomato and is published in Nat Neurosci. 2012 Mar 25;15(5):793-802. doi: 10.1038/nn.3078. This plasmid is available through Addgene.
WebReady-to-use AAV8 particles produced from pAAV-hSyn-hChR2 (H134R)-mCherry (#26976). In addition to the viral particles, you will also receive purified pAAV-hSyn … 10號公報 存貨之會計處理準則WebCAG-LSL-ChR2(H134R)-tdTomato-WPRE),orAi32(Rosa-CAG-LSL-ChR2(H134R)-EYFP-WPRE). The bred mice used in this ... Ai27, and SOM::Ai32 (SOM-ChR2), as well as PV::Ai27 and PV::Ai32 (PV-ChR2). Optogenetic Tagging and Manipulation of Cortical SOM and PV INs We first performed immunohistological experiments to assess the … 10蒸吨WebDec 1, 2024 · Mice of either sex expressing Cre-recombinase under the calretinin gene promotor (CAL-ires-Cre) were crossbred with Rosa-CAG-LSL-ChR2(H134R)-eYFP-WPRE (Ai32) mice, as described (Hibberd et al., 2024) (see Fig. 1 A).Resulting Cal Cre+ progeny expressed the ChR2(H134R)-eYFP fusion gene in calretinin-immunoreactive neurons. … tasti memeWebAi32 +/ mice, which contain ChR2(H134R)-EYFP downstream of a loxP-flanked STOP cassette to express ChR2 in a Cre-dependent manner (The Jackson Laboratory #024109), to produce Vglut2- 10荷兰WebWe used two strategies to express the light-activated ion channel, channelrhodopsin-2 (ChR2), in Piezo2 -expressing neurons: (i) ChR2 was constitutively expressed in cells from Piezo2 lineage by crossing Piezo2-GFP-IRES-Cre mice ( 21) to Ai32 mice (“constitutive Piezo2-ChR2 + mice”; Fig. 1A and fig. S1A) ( 22) and (ii) ChR2 was postnatally … 10袋×2合Webally expressing a ChR2(H134R)-tdTomato fusion protein, and Ai32, conditionally expressing a ChR2-EYFP fusion protein, have been de-scribed previously (Madisen et al., 2012). The Cre-driver and optoge-netic effector transgenic lines were backcrossed to C57BL/6NCrl, maintained separately on this genetic background, and interbred to 10%葡萄糖500ml加多少胰岛素WebOct 6, 2024 · The threshold and incidence of paw withdrawal and aversive behavior in a blue-lit room were dependent on ChR2 expression level; the strongest response was observed in NaV1.7iCre/iCre;Ai32/Ai32 mice. Thus, we developed a non-invasive pain model in which peripheral nociceptors were optically activated in free-moving transgenic … 10被5除