Cst myc antibody

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August undefined, 2024

WebMyc-Tag (9B11) Mouse mAb detects recombinant proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag fused to either the amino or carboxy terminus of … WebIP. ChIP. Chromatin immunoprecipitations were performed with cross-linked chromatin from K-562 cells and either MTA3 (E3X2E) Rabbit mAb or Normal Rabbit IgG #2729, using SimpleChIP ® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. The enriched DNA was quantified by real-time PCR, using human Myc exon 2 primers and SimpleChIP ® …

Can I use primary mouse antibodies on mouse tissue for ChIP?

WebApr 13, 2024 · DNA fragments were incubated overnight with specific antibody c-Myc (E5Q6W, CST, USA) and control IgG. The next day, antibody-binding magnetic beads were added. Proteinase K was used to elute and digest the immunoprecipitated product. The purified DNA product was used for subsequent PCR detection (Table S2). Mouse IgG … Web6x-His Tag Antibody (MA1-21315) in ICC/IF. Immunofluorescent analysis of 6x-His Tag was performed using 70% confluent HEK-293 cells transfected with V5-H3-His construct. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature ... rc car body painted

Anti-c-Myc (phospho T58) antibody (ab28842) Abcam

WebThe proto-oncogene MYC plays a role in human oncogenesis. For more information see here.. This recombinant rabbit monoclonal antibody (Y69) to c-Myc specifically detects … WebThe FLAG tag (peptide sequence DYKDDDDK) is a short, hydrophilic protein tag commonly used in conjunction with antibodies in protein pull-downs to study protein–protein interactions. The FLAG tag may be inserted at the N terminus, the N terminus preceded by a methionine residue, the C terminus, or internal positions of the target protein. WebC – Pulmonary EC co-transfected with GFP-tagged CLIP-170, CLASP2, EB1 or β-tubulin and Myc-SOCS1 were tested in co-IP assay with GFP antibody followed by immunoblotting with Myc-antibody. Probing of total cell lysates with GFP and Myc antibodies was used to verify ectopic protein expression; membrane re-probing for β-actin was used to ... sims 4 mods that make the game look better

Myc-Tag (9B11) Mouse mAb from Cell Signaling Technology

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WebMonoclonal Antibody for studying Myc. Cited in 820 publications. Validated for WB, WB, IF. Available in 2 sizes. Highly specific and rigorously … WebThe mouse Anti-c-MYC monoclonal antibody recognizes an epitope located within a sequence (EQKLISEEDL) of the product of the human oncogene c-myc. c-Myc (v-myc avian myelocytomatosis viral oncogene homolog) is a transcription factor, which is coded by MYC gene. It was first described as a homolog of an avian retroviral oncogene. rc car body stl filesWebWe hypothesized that Euonymus sachalinensis (ES) induces apoptosis by inhibiting the expression of c-Myc in colon cancer cells, and this study proved that the methanol extract of ES has anticancer effects in colon cancer cells. ES belongs to the Celastraceae family and is well known for its medicinal properties. Extracts of species belonging to this family … rc car builder near me

"WebFeb 2, 2007 · DOI: 10.1002/prot.22080. Primary Citation of Related Structures: 2OR9, 2ORB. PubMed Abstract: The X-ray structure of the Fab fragment from the anti-c-myc … " - Cst myc antibody

Cst myc antibody

WebAnti-c-Myc , Cat. No. 06-340-I, is a rabbit polyclonal antibody that detects c-Myc and is tested for use in Immunocytochemistry and Western Blotting. Physical form Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide WebImmunofluorescent analysis of Transfected HEK-293 cells using 66004-1-Ig (MYC-tag antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L). This product is out of stock now, and we suggest you go to the replacement 60003-2-Ig. Our concentration is detected with BSA as standard.

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WebICC staining of purified anti-c-Myc antibody (clone 9E10) on HeLa cells transfected with multi-tag plasmid containing c-Myc epitope sequence. The cells were fixed with 4% PFA, permeabilized with a buffer containing 0.1% Triton X-100, and blocked with 5% fetal bovine serum in PBS for 1 hour. The cells were then incubated with 1 µg/mL of the ... Webc-Myc (also known as Myc), together with l-Myc and n-Myc, belongs to the Myc family of transcription factors. c-Myc has a basic helix-loop-helix leucine zipper domain and through heterodimerization can bind and regulate the transcriptional activity of genes, either by repression or activation. It is a key player in the regulation of cell growth ...

Webc-Myc Antibody. Applications: WB, IHC, ICC/IF, IP, ELISA. Reactivity: Human, Mouse, Rat, Tag, Feline. Publications: 749. Images: 417. ... The c-Myc protein is a 62 kDa … WebCST complex. The CST complex is a cellular multiprotein complex involved in telomere maintenance. In budding yeast ( Saccharomyces cerevisiae ), it is composed of the …

WebCells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 3% BSA for 1 hour at 22°C. Samples were incubated with primary antibody (1/1000 in diluent) for 1 hour at 22°C. A FITC-conjugated goat anti-mouse polyclonal IgG (1/1000) was used as the secondary antibody. Nuclei were stained with DAPI (blue). See ... WebGet better batch-to-batch reproducibility with a recombinant antibody. Anti-c-Myc (phospho T58) antibody [EPR17923] (ab185655) Research with confidence – consistent and reproducible results with every batch. Long-term and scalable supply – powered by recombinant technology for fast production.

WebAt CST, we adhere to the Hallmarks of Antibody Validation™, six complementary strategies for determining the specificity, sensitivity, and functionality of an antibody in any given assay. By carefully tailoring these strategies to each antibody product, we guarantee that CST antibodies will work as expected, to help you achieve results you ...

WebACTL6B/BAF53B is a member of the neural-specific SWI/SNF complex that facilitates binding to target genes and is involved in memory and synaptic plasticity (13-15). ACTL6/BAF53 has been shown to interact with c-Myc, where it functions as a cofactor and is important in the transformation process (16). Further studies have shown … sims 4 mods that need to be updated 2023WebMouse monoclonal c-Myc antibody [8]. Validated in IHC and tested in Human. Cited in 6 publication(s). Independently reviewed in 6 review(s). Immunogen corresponding to recombinant full length protein. sims 4 mods that fix the gameWeb13 hours ago · Our previous studies demonstrated that rapamycin and rapalogs significantly induced IRES-mediated translation of cyclin D1 and c-myc mRNAs following exposure [16, 20, 21].The ability of these compounds to induce IRES activity of these two determinants was sufficient to render cells resistant to rapamycin in an AKT-dependent manner … rc car cake ideasWebDec 4, 2015 · The antibody is question is the cell signaling Myc-tag antibody and I am hoping to use mouse tissues in an upcoming ChIP experiment. ... It might be more important to have a very nice anti-MYC ... rc carbon fiber motorcycle partsWebThe c-Myc Antibody from Cell Signaling Technology is a Rabbit Polyclonal antibody to MYC. This antibody recognizes Human, Mouse, and Rat antigen. The c-Myc Antibody … rc car champWebThe c-Myc protein is a 62 kDa transcription factor that is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous ... rc car burnoutWebc-Myb Antibody (D-7) is a mouse monoclonal IgG 2a κ c-Myb antibody, cited in 19 publications, provided at 200 µg/ml. c-Myb Antibody (D-7) is recommended for detection … rc car builds