Hif1a antibody

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August undefined, 2024

Web11 de nov. de 2015 · Abstract. Background: Hypoxia Inducible Factor (HIF) regulates a cascade of transcriptional events in response to decreased oxygenation, acting from the cellular to the physiological level. This response is evolutionarily conserved, allowing the use of zebrafish (Danio rerio) as a model for studying the hypoxic response. WebHIF1A Antibodies. Antibodies that detect HIF1A can be used in several scientific applications, including Immunohistochemistry, Western Blot, Immunocytochemistry, Flow …

HIF-1alpha/HIF1A Mouse Monoclonal Antibody (HIF-1alpha/HIF1A …

WebAnti-HIF1A antibody produced in rabbit (Anti-ARNT-interacting protein ); Prestige Antibody powered by Atlas, affinity isolated antibody, buffered aqueous glycerol solution; Suitable for immunohistochemistry; Rabbit polyclonal anti-HIF1A antibody is used to tag hypoxia-inducible factor 1 α for d WebClone 54/HIF-1α. Western blot analysis of HIF-1α on lysate from HeLa stimulated with CoCl2, cobalt chloride. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the Mouse … how is soil evidence collected

HIF-1alpha/HIF1A Mouse Monoclonal Antibody (HIF-1alpha/HIF1A …

WebShowing subcellular location of HIF1A (bHLHe78, HIF-1alpha, HIF1, MOP1, PASD8). We use cookies to enhance the usability of our website. ... Location antibody staining i. Location tagged protein i HPA000907: HeLa: 5803: N-terminal: Nucleoplasm, Plasma membrane: Nucleoplasm, Plasma membrane ... WebA specific band was detected for HIF-1 alpha/HIF1A at approximately 116 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse/Rat HIF-1 alpha/HIF1A Monoclonal … WebView our 36 HIF-1 alpha/HIF1A products for your research including HIF-1 alpha/HIF1A Primary Antibodies, Small Molecules, Proteome Profiler Antibody Arrays, Peptides, ELISAs, and cDNA Clones. how is soil compaction measured

HIF1-alpha Polyclonal Antibody (A300-286A) - Thermo Fisher …

Purified Mouse Anti-Human HIF-1α - BD Biosciences

WebThis antibody does not cross-react with ARNT or the related HIF-2 alpha. MA1-516 has been successfully used in Western blot, immunofluorescence, immunoprecipitation, … WebHIF1A Antibody detects endogenous levels of total HIF1A. RRID: AB_2835328 Cite Format: Affinity Biosciences Cat# AF1009, RRID:AB_2835328. Conjugate: Unconjugated. Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific). Storage: ... how is soil defined by soil scientistsWebI am doing HIF1 westerns in HIF-overexpressing mouse liver and adipose tissue using Novus antirabbit HIF1a antibody with overnight incubation. I am getting strong bands around 90kDa. I am aware that HIF theoretical molecular weight is 93kDa, but in westerns, the HIF band is usually around 120kDa according to my internet research. how is soil health measured

"WebAlternative Name: HIF1; HIF-1A; HIF1A; HIF-1-alpha; HIF1-alpha; BHLHE78; MOP1; PASD8 Reactivity: Human (QC Testing) Isotype: Mouse IgG1, κ ... Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. " - Hif1a antibody

Hif1a antibody

Genome-wide mapping of Hif-1α binding sites in zebrafish

WebHIF-1alpha/HIF1A Mouse Monoclonal Antibody -20ºC保存，需避免反复冻融。 Western一抗稀释液-20℃或4℃保存，一年有效，Western一抗稀释液优先推荐4℃保存，长期不使 … Web丁香通为您提供PhosphoHIF1A商品详情介绍：价格：￥2200 - 2800，货号：AF0062，品牌：Affinity Biosciences，详见丁香通PhosphoHIF1A商品详情页；

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WebWestern blot - HIF1α Rabbit pAb (A11945) Western blot analysis of extracts of HeLa cells, using HIF1α antibody (A11945) at 1:1000 dilution.Hela cells were treated by Cobalt chloride (0.1 mM) at 37℃ for 4 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. WebThis antibody is directed against human HIF-1a hydroxyP564 and is specific for the hydroxylated form of the protein. Minimal reactivity occurs with the non-hydroxylated form of the protein. A BLAST analysis was used to suggest cross-reactivity with HIF-1a from human, monkey, mouse, rat, dog, bovine and Xenopus sources based on a 100% homology ...

WebSequence. Sequence: Please enter your sequence in the 5' to 3' direction. Sequences must be composed solely of the nucleotides A, C, G, T. Please convert U's to T's and remove … WebMonoclonal Anti-HIF-1α antibody produced in mouse (Anti-Hypoxia Inducible Factor 1, α subunit); ~ 1mg/mL, monoclonal H1α67, purified immunoglobulin, buffered aqueous solution; ... Anti-HIF1A. affinity isolated antibody. View …

WebHIF1A Antibody (PA5-85494) in ICC/IF. Immunofluorescence analysis of Hypoxia-inducible factor 1-alpha was performed using 70% confluent log phase MCF7 cells treated with 150 µM of Cobalt chloride for 16 hours. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with ... WebPolyclonal Antibody for studying HIF1A. Cited in 194 publications. Validated for Western Blotting. Highly specific and rigorously validated in …

WebHIF-1 alpha antibody used for WB on lysates of HUVEC/human umbilical vein endothelial cells after exposure to 2 Gy of IR with or without 2 day pre-incubation with …

WebDetection of human HIF1 alpha by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from 293T cells that were treated with 200 µM cobalt chloride.Antibodies: Affinity purified rabbit anti-HIF1 alpha antibody A300-286A (lot number A300-286A-6) used for WB at 1 µg/ml and for IP at 6 µg/mg lysate.Detection: … how is solar energy developedWebThis antibody does not cross-react with ARNT or the related HIF-2 alpha. MA1-516 has been successfully used in Western blot, immunofluorescence, immunoprecipitation, immunocytochemistry, and gel shift procedures. By Western blot, this antibody detects an ∽116 kDa protein representing HIF-1 alpha after hypoxic induction in COS cells. how is soil useful to usWebUnder hypoxic conditions, HIF-1 activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular … how is solar electricity storedWebHIF1a/HIF-1 alpha antibody used for WB on proteins extracts from human lymphoblasts cultured under normoxic and hypoxic conditions. Lymphoblasts were from six patients … how is soil tested how is soil related to bedrockWebHIF1-alpha (HIF1A) is a subunit of HIF1, which is a transcription factor found in mammalian cells cultured under reduced oxygen tension. HIF-1 is a heterodimer consisting of an … how is solar energy exploitedWeb13 de mai. de 2024 · DNA was immunoprecipitated from the sonicated cell lysates using anti-HIF1A antibody (Abcam) and immunoprecipitates were recovered by addition of Salmon Sperm DNA/Protein A Agarose-50% Slurry. how is soil texture determined