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Phic31 attp

WebINTRODUCTION. The Streptomyces phage phiC31 uses a large serine recombinase, phiC31 integrase (Int), to merge its genome with that of its bacterial host [1, 2].Its two substrates … http://www.hbaas.com/xumusuo/view/15912.html

Site-specific chromosomal integration mediated by phiC31 integrase

WebThe enzyme efficiently mediates recombination between two distinct sequences, attP and attB, producing recombinant product sites, attL and attR. The reaction proceeds … WebBinary vector pCOXS3-phiC31 Binary vector pCRB_mpt0 Binary vector pCRB_PMpHSP17.8 Binary vector pCS Binary vector pCS4-BASK Binary vector pCsGFPBT Binary ... Cloning vector pBXL_attP_PUbDsRed.T3_fa_s1-tTA_a Cloning vector pC-(lox2-attB2-SA-Hsp70)3 Cloning vector pC-(loxP2-attB2-SA(0)) mckids happy meal https://lamontjaxon.com

A Gateway MultiSite Recombination Cloning Toolkit

Web15. okt 2024 · DH5 onset competent cells (Zymo). Clones which selected by ampicillin resistance on Amp-LB shelves (60 mg/mL). Purified plasmids have injected at 500 ng/uL into aforementioned phiC31 integrase-expressing 86Fb jetty site lines BDSC 24749 (courtesy of Rainbow Transgenics) for phiC31 attP-attB integration and sifted for using a mini-white … Web20. aug 2024 · PhiC31 integrase-based vectors can integrate therapeutic genes selectively into attP or pseudo-attP sites in genomes, but considerable numbers of pseudo-attP … Web14. jan 2024 · PhiC31 integrase-mediated transgenesis systems are based on the site-specific bacteriophage PhiC31 integrase which mediates sequence-directed, irreversible … mckie ford lincoln - rapid city

Addgene: pUPD2 PhiC31 attP:TMtb:P35S:attB (GB1494)

Category:Site-specific recombinase technology - Wikipedia

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Phic31 attp

多模块生物合成酶基因组合文库的制备方法【掌桥专利】

WebThe PhiC31 integrase is a sequence-specific recombinase encoded within the genome of the bacteriophage PhiC31. The PhiC31 integrase mediates precise, unidirectional recombination between two att sites named attB and attP. This integrase has been shown to function efficiently in many different cell types as well as in mice [12, 13].

Phic31 attp

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WebDuring pUAST/PhiC31 integrase-mediated integration, the unique attP site in the ZH-attP-51D fly line genome recombines with the pUAST attB site in the pUAST-PrP vector forming … Webmedia.addgene.org ... SnapGene ...

WebBackbone manufacturer Stratagene Vector type Mammalian Expression, Bacterial Expression, Mouse Targeting ; att/PhiC31 Selectable markers Neomycin (select with G418) Growth in Bacteria Bacterial Resistance (s) Ampicillin, 100 μg/mL Growth Temperature 30°C Growth Strain (s) DH10B Copy number High Copy Gene/Insert Gene/Insert name Neo* … WebphiC31 recombinase through orthogonal riboregulated systems. The example gate shown implements AND functionality. The performance of this gate was characterized by the percentage of cells that were GFP+ as assayed using flow cytometry after gating by forward and side scatter, and after exposure to the indicated set of inputs.

Web21. júl 2006 · The stability of the attB × attP locus, well-documented with the phiC31 integrase system in bacteria and yeast 16,17, was evidenced here by the lack of … Web17. jún 2010 · PhiC31 integrase induces intramolecular recombination between target sites in episomal DNA in zebrafish. a Schematic of the reporter construct Tg(eab2:attP …

Web25. júl 2024 · (A) The H11 locus was cleaved, using CRISPR/Cas9, to encourage integration of the landing pad donor. A successful knock-in at the H11 locus generates a master cell …

WebpJTI TM Phic31 Int载体不含NLS信号。加入NLS信号可能会增加假attP位点的特异性整合效率，但尚未获得支持数据。有一篇文献描述了在PhiC31整合酶载体上NLS信号的应用，但作 … lichens of the northwoodshttp://www.biofeng.com/zaiti/qita/pSET152.html mckibbon law firm - greenville scWebThermo Fisher Scientific is dedicated to improving the human condition through systems, consumables, and services for researchers. lichens of new yorkWebAlthough meganucleases, zinc-finger- and transcription activator-like effector nucleases (ZFNs and TALENs) are actual tools supporting HR, it was the availability of site-specific … lichens of finlandWeb23. mar 2024 · 该研究一个重要的创新点是通过改造酵母双杂交系统的载体质粒(Yang et al., 2024), 将诱饵蛋白所在载体(pGBKT7)插入ATTB位点, 将捕获蛋白所在载体(pGADT7)插 … mckie certified pre ownedWebPhiC31整合酶可以识别特异序列attP（噬菌体结合位点）和attB（细菌结合位点），使两段序列之间发生高效且不可逆的整合反应。将含有attB序列和w+ 筛选标记的质粒注射入 … lichens of ohioWeb湖北省农业科学院是省政府直属事业单位，是种植业、畜牧业、特产业相结合的综合性农业研究机构，是全省农业科学的最高学术机构和研究中心。 其主要职责是： (一)组织院属各研究单位开展农业科学的应用研究和应用基础研究，提高我省的农业科学技术水平。 (二)组织院属各单位对我省农业资源、生态资源等进行综合性考察、研究、试点，积累科学资料，为国 … lichens of ontario