Phocl variant
WebbWe demonstrated that PhoCl can be used to engineer light-activatable Cre recombinase, Gal4 transcription factor, and a viral protease that in turn was used to activate opening … WebbcPass™ Technology. SARS-CoV-2 Surrogate Virus Neutralization Test (sVNT) Kit (RUO) cPass™ SARS-CoV-2 Neutralization Antibody Detection Kit (FDA EUA)
Phocl variant
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Webb13 mars 2024 · An engineered photoconvertible fluorescent protein (pcFP) variant, designated as mMaple, that is suited for use in multiple conventional and super … Webb10 dec. 2024 · 40 summary reveals, PhoCl is the sole member of a distinct class of optogenetic actuators and 41 uniquely enables irreversible optogenetic activation via a …
WebbSeveral different screening systems based on bimolecular fluorescence complementation and FRET were developed to screen directly for the photoinduced dissociation of a photocleavable protein (PhoCl) developed in our lab. A second photocleavable protein called SplitOr was created from PSmOrange2.
WebbThe improved performance of PhoCl2c is also made apparent by the fact that this variant induced a greater degree of capase-3 activation compared to PhoCl1 (Fig. 5F). Cell … DNA encoding the NES of MAPKK (NLVDLQKKLEELELDEQQ)26 and the NLS of SV40 (PKKKRKV)27were separately appended by PCR to the 5′ end of the gene encoding PhoCl. The gene encoding mCherry was inserted between the KpnI and XhoI sites of pcDNA3.1(+) (Thermo Fisher Scientific). Genes encoding NES … Visa mer Plasmid construction was performed through standard restriction-enzyme cloning. Pfu (Thermo Fisher Scientific), Taq (New England BioLabs), and Q5 (New England BioLabs) DNA polymerases were used for polymerase … Visa mer Overlap PCR was used to circularly permute the gene encoding mMaple, such that the original termini were joined with a GGSGG linker, and new … Visa mer All cell lines were grown from original frozen stocks without authentication or mycoplasma detection. The HEK cell line is listed in the International Cell Line Authentication … Visa mer The genes for mMaple, PhoCl, and PhoCl-MBP in pBAD/His B were used to transform E. coli strain DH10B or Top10. LB medium supplemented with 100 μg/mL ampicillin was inoculated with these transformants, and the … Visa mer
WebbPhoCl has since been used by other labs to generate tunable hydrogels, 23 release proteins from biomaterials, 24, 25 study cell mechanotransduction, 26 and generate …
Webb31 maj 2024 · PhoCl variants: PhoCl2c with higher dissociation contrast ratio, and PhoCl2f with faster dissociation rate. Optogenetic control of protein localization by PhoCl2 variants fixing hooded eyesWebbPhoCl protein was engineered by Campbell group in 2013 ( Zhang et al., 2024 ). Upon the illumination of violet light (~400 nm), the chromophore undergoes a β-elimination reaction, leading to the cleavage of the polypeptide backbone. The two fragments of the protein then spontaneously dissociate and become unstructured. fixing hooded lidsWebbPhotocleavage leads to redistribution of a red FP (mCherry) between the cytoplasm and nucleus. Relative to PhoCl1-mCherry, PhoCl2c-mCherry fusion exhibited a more even red … fixing hooded eyes with botoxWebb9 feb. 2024 · These proteins contain the chromophore retinal, or a variant, which in response to light isomerizes between an 11- or 13-cis and all-trans retinal. ... Fluorescent proteins such as PhoCl, PYP or Dronpa, which were also used to implement light control are not discussed further. fixing hollow tile floorsWebbA non-protease-based optogenetic technique termed PhoCl (PhotoCleavable protein) based on violet-light-induced β-elimination reaction of a photoconvertible uorescent … can my hamster have applesWebb9 dec. 2024 · Here, we report the X-ray crystal structures of the PhoCl1 green state, red state, and cleaved empty barrel. Molecular dynamics (MD) simulations were performed to provide insight into the precise dissociation mechanism. Using structure-guided engineering and directed evolution, we have developed PhoCl2c with higher contrast … fixing hoof flareWebbThe finalized PhoCl system has a broad absorbance from 400 to 500 nm, and the dissociation rate is 500 s. Many proteins, ≈ such as Cre recombinase, Gal4 transcription factor, Pannexin-1 ion channel, could be controlled by PhoCl.[10] can my hardwood floors be refinished