Web24 Feb 2024 · The whiskers act as assembly jigs for adding the long tail fibers to the virion, and they also act as environmental sensors that sequester the long tail fibers under … Plasmid expression vectors containing the tfibMu and tfaMu genes were expressed in E. coli BL21(DE3)pLysS cells in M9 medium containing a native amino-acid mixture. Selenomethionine was also added to the mixture to produce selenomethionyl-labelled proteins. The produced complex was further purified as described … See more The genes tfibMu and tfaMu and segments thereof were amplified by PCR from genomic phage Mu DNA. The tfibMu fragments were cloned into a derivative of … See more Purified full-length His6–TfibMu:TfaMucomplex was negatively stained with 2% (w/v) uranyl acetate on 400-mesh copper grids coated with carbon. Microscopy … See more Phage titres were determined using a plaque assay. A 150 μl volume of an overnight culture of E. coli strain QD5003, an amber suppressor strain (supF), was added … See more LPS extraction was performed as previously described46. One hundred micrograms of bacterial cell pellet was resuspended in 5 ml of distilled deionized water, … See more
Viral tail assembly ~ ViralZone - Expasy
Webin the proximal long tail fiber protein gp34 (Granell et al., 2024). In contrast to bacteriophages, the T6SS does not have tail fibers. ... Assembly of the tail of bacteriophage T4. J Supramol Struct 3: 24–38. Kikuchi, Y., and King, J. (1975b) Genetic control of bacte-riophage T4 baseplate morphogenesis. I. Sequential assembly of the major ... Web7 Dec 2024 · The tail formation is completed by the binding of six gp17 fiber protein trimers, which are responsible for host cell recognition, onto the gp11–gp12 interface (12. Hu B. … great gama routine
Phage Proteins Required for Tail Fiber Assembly Also Bind ... - PubMed
WebTail fiber assembly protein Gene 38 Status UniProtKB reviewed (Swiss-Prot) Organism Enterobacteria phage T4 (Bacteriophage T4) Amino acids 183 Protein existence Inferred … WebTail tube protein polymerization starts from baseplate assembly. The tail measure protein possibly assembles with tail assembly chaperones, and the resulting complex interacts … Web14 Oct 2024 · The proper folding and assembly of tail fibers usually requires a chaperone encoded by the phage genome. Despite extensive studies on phage … At the first step of … flithgs from long island to west virginia